Transcription profiling of Arabidopsis dst1 mutants

ABSTRACT: In this study, DNA microarray analysis was used to expand our understanding of the dst1 mutant of Arabidopsis. The dst (downstream) mutants were isolated originally as specifically increasing the steady state level and the half-life of DST-containing transcripts. As such, they offer a unique opportunity to study rapid sequence-specific mRNA decay pathways in eukaryotes. These mutants show a threefold to fourfold increase in mRNA abundance for two transgenes and an endogenous gene, all containing DST elements, when examined by RNA gel blot analysis; however, they show no visible aberrant phenotype. Here, we use DNA microarrays to identify genes with altered expression levels in dst1 compared with the parental plants. In addition to verifying the increase in the transgene mRNA levels, which

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4010 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress