Virtual northern on human brain samples using DNA microarrays for systematic genome wide analysis of mRNA lengths

ABSTRACT: Background We applied the Virtual Northern technique to human brain mRNA to systematically measure human mRNA transcript lengths on a genome-wide scale. Methodology/Principal Findings We used separation by gel electrophoresis followed by hybridization to cDNA microarrays to measure 8,774 mRNA transcript lengths representing at least 6,238 genes at high (>90%) confidence. By comparing these transcript lengths to the Refseq and H-Invitational full-length cDNA databases, we found that nearly half of our measurements appeared to represent novel transcript variants. Comparison of length measurements determined by hybridization to different cDNAs derived from the same gene identified clones that potentially correspond to alternative transcript variants. We observed a close linear relationship

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress