Transcription profiling of Streptomyces coelicolor mutant unable to erect aerial hyphae identifies a previously unknown layer of erial mycelium surface proteins (the "chaplins")

ABSTRACT: The filamentous bacterium Streptomyces coelicolor differentiates by forming specialized, spore-bearing aerial hyphae that grow into the air. Using microarrays, we identified genes that are down-regulated in a mutant unable to erect aerial hyphae. Through this route, we identified a previously unknown layer of aerial mycelium surface proteins (the "chaplins"). The chaplins share a hydrophobic domain of ~40 residues (the "chaplin domain"), and all have a secretion signal. The five short chaplins (ChpD,E,F,G,H) have one chaplin domain, whereas the three long chaplins (ChpA,B,C) have two chaplin domains and a C-terminal "sorting signal" that targets them for covalent attachment to the cell wall by sortase enzyme. Expression of the two chaplin genes examined (chpE, chpH) depended on aerial hy

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress