Transcription profiling of Arabidopsis plants treated with trichostatin A to block histone deacetylation or 5-aza-2-deoxycytosine to block cytosine methylation

ABSTRACT: Blocking histone deacetylation with trichostatin A (TSA) or blocking cytosine methylation using 5-aza-2'-deoxycytosine (aza-dC) can derepress silenced genes in multicellular eukaryotes, including animals and plants. We questioned whether DNA methylation and histone deacetylation overlap in the regulation of endogenous plant genes by monitoring changes in expression of ~7800 Arabidopsis thaliana genes following treatment with azadC, TSA, or both chemicals together. RNA levels for ~4% of the genes were reproducibly changed 3-fold or more by at least one treatment. Distinct subsets of genes are up-regulated or down-regulated in response to aza-dC, TSA, or simultaneous treatment with both chemicals, with little overlap among subsets. Surprisingly, the microarray data indicate that TSA and aza-

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress