Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of yeast ET/Calcium Time Series

ABSTRACT: Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing ET and 0.4M CaCl2 was added to the ET-treated culture (final concentration 0.2 M CaCl2). Cells were collected by centrifugation at 5, 15, 30 and 60 min, frozen at 80oC and processed for RNA extraction. For the publication, the experiment was repeated twoce, and corresponding timepoints from each experiment were averaged. The first 4 and last 4 arrays in this set correspond to each experiment.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hiroyuki Yoshimoto 

PROVIDER: E-SMDB-7 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.

Yoshimoto Hiroyuki H   Saltsman Kirstie K   Gasch Audrey P AP   Li Hong Xia HX   Ogawa Nobuo N   Botstein David D   Brown Patrick O PO   Cyert Martha S MS  

The Journal of biological chemistry 20020610 34

In Saccharomyces cerevisiae, the Ca(2+)/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca(2+) and Na(+), and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca(2+) (200 mm) or Na(+) (0.8 m) to yeast. 163 genes exhibited increased expression that was reduced 50%  ...[more]

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