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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of cell cycle in HeLa S3 cells, double thymidine Block, experiment 1

ABSTRACT: HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the first double thymidine block synchronization (Thy-Thy1).

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter

PROVIDER: E-SMDB-901 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes periodically expressed in the human cell cycle and their expression in tumors.

Whitfield Michael L ML Sherlock Gavin G Saldanha Alok J AJ Murray John I JI Ball Catherine A CA Alexander Karen E KE Matese John C JC Perou Charles M CM Hurt Myra M MM Brown Patrick O PO Botstein David D

Molecular biology of the cell 20020601 6

The genome-wide program of gene expression during the cell division cycle in a human cancer cell line (HeLa) was characterized using cDNA microarrays. Transcripts of >850 genes showed periodic variation during the cell cycle. Hierarchical clustering of the expression patterns revealed coexpressed groups of previously well-characterized genes involved in essential cell cycle processes such as DNA replication, chromosome segregation, and cell adhesion along with genes of uncharacterized function. ...[more]

PMID: 12058064

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Transcription profiling of cell cycle in HeLa S3 cells, double thymidine block, rxperiment 2

Project description:HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the second double thymidine block synchronization (Thy-Thy2).

2005-09-22 | E-SMDB-902 | biostudies-arrayexpress

Transcription profiling of cell cycle in HeLa S3 cells, double thymidine block, experiment 3

Project description:HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the third double thymidine block synchronization (Thy-Thy3).

2005-09-22 | E-SMDB-903 | biostudies-arrayexpress

Transcription profiling of cell cycle in HeLa S3 cells, thymidine-nocodazole lock

Project description:HeLa S3 cells were synchronized in mitosis by arresting first in S phase with thymidine followed by arrest in mitosis with Nocodazole as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the thymidine-nocodazole synchronization (Thy-Noc).

2005-09-22 | E-SMDB-904 | biostudies-arrayexpress

Cell division cycle in a human cancer cell line (HeLa)

Project description:This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2005-10-22 | GSE3497 | GEO

Cell division cycle in a human cancer cell line (HeLa)

2005-10-22 | E-GEOD-3497 | biostudies-arrayexpress

HeLa cell cycle time series - double thymidine block

Project description:HeLa cell cycle time series - double thymidine block

2012-05-05 | E-TABM-1155 | biostudies-arrayexpress

RNA sequencing on HT29 colorectal cancer cell line

Project description:We report the single cell RNA sequencing on HT29 colorectal cancer line with 100 nM dasatinib treatment after double thymidine block.

2020-09-30 | GSE128416 | GEO

Identification of the cell cycle specific proximal interactors of PCDH7

Project description:We used proximity dependent biotin identification (BioID) method to determine cell cycle specific interaction partners of PCDH7. HeLa S3 cells were synchronized to interphase and mitosis using double tymidine block and double thymidine block followed by S-Trityl-L-cysteine (STC) treatment respectively. Candidate interactors were isolated using streptavidin affinity purification and identified using LC-MS/MS analysis.

2023-02-27 | PXD029246 | Pride

Cell cycle expression profiles in HeLa cells

Project description:Periodic expression of cell cycle genes highlights the importance of precise temporal control of transcription in regulating cell cycle events. We used HeLa cells enriched for different phases of the cell cycle to identify genes that are expressed in a periodic fashion in specific cell cycle phases. These data were generated for comparison with ChIP-Sequencing data obtained for two subunits of the B-Myb-MuvB complex, B-Myb and LIN9, in HeLa cells. HeLa cells were synchronized at the beginning of S phase by double thymidine block and then released into the cell cycle by washing out the thymidine resulting in tight synchrony at S, G2 and M phases of the cell cycle. Most cells entered mitosis at 8 hours after release as determined by visual inspection. Three independent replicas of double thymidine block and release experiments were performed. RNA was isolated at specific times after release from the thymidine block (0, 2, 4, 6, 8 and 12 hours) and used for generating expression profiles.

2012-01-25 | E-GEOD-26922 | biostudies-arrayexpress

Identification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.

Project description:HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. After a release of 7.5 h in fresh medium, DMSO or G6PD inhibitor was added to the medium. 1 h later, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, the samples were subjected to LC-MS/MS analysis. Finally, a Kinase-Substrate Enrichment was performed, which could infer the changes of upstream kinase activity upon the treatment of G6PD inhibitors.

2023-05-18 | PXD033825 | Pride

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