Transcription profiling of fission yeast meiosis and sporulation

ABSTRACT: pat1 meiosis is very synchronous (see E-SNGR-2), but the use of a temperature shift and the prolonged nitrogen starvation can lead to artifacts. As a control for the pat1 experiment,non-sporulating pat1+ diploids were treated exactly as in the protocol for pat1 cells, with the exception that no nitrogen was added at the time of the temperature shift. Under these conditions the cells remain blocked in G1. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Cordelia Langford 

PROVIDER: E-SNGR-6 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress