Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of liver, skeletal muscle and white adipose tissue from ApoE3L mice

ABSTRACT: Our aim was to investigate gene expression changes over time in liver of ApOE3*Leiden mice on a high-fat diet. Probesets were remapped and annotated into Entrez gene-ids using the custom MBNI CDF-file, version 9.0.1 - the GCRMA data generated

ORGANISM(S): Mus musculus

SUBMITTER: Marjan van Erk

PROVIDER: E-TABM-1039 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance.

Kleemann Robert R van Erk Marjan M Verschuren Lars L van den Hoek Anita M AM Koek Maud M Wielinga Peter Y PY Jie Annie A Pellis Linette L Bobeldijk-Pastorova Ivana I Kelder Thomas T Toet Karin K Wopereis Suzan S Cnubben Nicole N Evelo Chris C van Ommen Ben B Kooistra Teake T

PloS one 20100121 1

<h4>Background</h4>The sequence of events leading to the development of insulin resistance (IR) as well as the underlying pathophysiological mechanisms are incompletely understood. As reductionist approaches have been largely unsuccessful in providing an understanding of the pathogenesis of IR, there is a need for an integrative, time-resolved approach to elucidate the development of the disease.<h4>Methodology/principal findings</h4>Male ApoE3Leiden transgenic mice exhibiting a humanized lipid ...[more]

PMID: 20098690

Similar Datasets

Project description:Because of the epidemic rise of obesity worldwide, the identification of novel target genes for pharmacological treatment of obesity and related disorders is becoming of high importance. IFRD1 and IFRD2 are members of a novel transcriptional regulators family. Intestinal over-expression of mouse homologue of IFRD1 promoted intestinal triglyceride uptake and induced whole body adiposity in mice. To further elucidate the role of IFRD1 and IFRD2 in vivo, we generated mice lacking both mouse homologues of IFRD1 (TIS7) and IFRD2 (SKMc15) genes. Here, we report that mice deficient in TIS7 and SKMc15 genes, despite normal calorie intake had severely reduced amount of adipose tissue, were resistant to diet-induced obesity and displayed high glucose tolerance. Lower dietary fat entry into the circulation suggested that this phenotype resulted from impaired intestinal lipid transport. We identified down-regulation of CD36, a fatty acid transporter, both on RNA and protein levels. Reporter assays indicated that TIS7 and SKMc15 transcriptionally regulated CD36 expression and CD36 overexpression partially restored fatty acid uptake in vitro. Hence, our study suggested that TIS7 and SKMc15 play an important role in the regulation of the lipid metabolism and might represent a novel strategy for treatment of disorders caused by excess fat intake. To determine whether decreased intestinal lipid absorption might be caused by changes in expression of lipid processing and transport molecules, we performed Affymetrix microarray analyses of total RNA samples isolated from the jejunum of HFD-fed WT type and dKO animals. The moderated t-test was used to calculate p-values for significance of differential gene expression between 3 dKO and 3 wild type mice. These raw p-values were adjusted for multiple hypothesis testing using the method from Benjamini and Hochberg for a strong control of the false discovery rate (FDR) and genes with thus adjusted p-values < 0.05 were considered significant. Age-matched (7-10 week old) male wild type and TIS7 (Ifrd1) SKMc15 (Ifrd2) double knock out mice (C57Bl6 background) were caged individually and maintained from 3 weeks up to 8 weeks on a synthetic high saturated fat (HFD) diet (Ssniff). Small intestines (jejunum) were harvested for total RNA isolation. RNAs from 3 WT and 3 dKO mice were subjected to Affymetrix based whole genome gene expression analysis (Mouse 430.2 GeneChip).

Dataset Information

Transcription profiling of liver, skeletal muscle and white adipose tissue from ApoE3L mice

Publications

Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure