Transcription profiling of human knee synovial biopsies before and after anti-TNF treatment in patients suffering from rheumatoid arthritis

ABSTRACT: The purpose of this experiment was to do investigatge the transcriptional effect of anti-TNF treatment in patients suffering from rheumatoid arthritis. Two series of hybridizations were performed. Each hybridization was done with a technical replicate (the same amount of RNA taken from the same amplified RNA aliquot labeled in two separate reactions and hybridized onto two separate arrays). In the first series of hybridizations RNA exptracted from biopsies taken before treatment was hybridized in a common reference design to enable comparisons between patients. In the second series RNA from biopsies taken after and before treatment were hybridized on the same chip in a direct design to enable investigation of the effect of treatment. 10 patients (8 females and 2 males, median age 54, range

INSTRUMENT(S): G2565BA DNA microarray scanner

ORGANISM(S): Homo sapiens

DISEASE(S): rheumatoid arthritis

SUBMITTER: Johan Lindberg 

PROVIDER: E-TABM-104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress