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Transcription profiling of human node-negative breast cancer patients to validate a 70-gene prognostic signature on a high-throughput microarray

ABSTRACT: A 70-gene signature has been shown to be a powerful predictor of disease outcome in young breast cancer patients. The microarrays were not designed for high throughput processing. To facilitate the use in a diagnostic setting, the profile was translated into a customized microarray containing a reduced set of 1,900 probes.

ORGANISM(S): Homo sapiens

SUBMITTER: Annuska Glas

PROVIDER: E-TABM-115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Converting a breast cancer microarray signature into a high-throughput diagnostic test.

Glas Annuska M AM Floore Arno A Delahaye Leonie J M J LJ Witteveen Anke T AT Pover Rob C F RC Bakx Niels N Lahti-Domenici Jaana S T JS Bruinsma Tako J TJ Warmoes Marc O MO Bernards René R Wessels Lodewyk F A LF Van't Veer Laura J LJ

BMC genomics 20061030

<h4>Background</h4>A 70-gene tumor expression profile was established as a powerful predictor of disease outcome in young breast cancer patients. This profile, however, was generated on microarrays containing 25,000 60-mer oligonucleotides that are not designed for processing of many samples on a routine basis.<h4>Results</h4>To facilitate its use in a diagnostic setting, the 70-gene prognosis profile was translated into a customized microarray (MammaPrint) containing a reduced set of 1,900 prob ...[more]

PMID: 17074082

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