Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of in vivo immune responses to mycobacteria using the tuberculin skin test

ABSTRACT: Transcriptional profiling of biopsies from tuberculin skin test sites was performed to investigate the interaction of complex cellular and molecular networks at the interface between innate and adaptive immunity which coordinate anti-mycobacterial responses in vivo.

ORGANISM(S): Homo sapiens

SUBMITTER: Gillian Tomlinson

PROVIDER: E-TABM-1157 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Delta lgt paper

Project description:We have used a lipoprotein-deficient mutant Streptococcus pneumoniae strain, delta lgt, to investigate the role of lipoproteins during inflammatory responses to live S. pneumoniae.

2015-01-31 | E-MTAB-1541 | biostudies-arrayexpress

Inhibition of macrophage IL10 responses by HIV 1

Project description:Inhibition of macrophage IL10 responses by HIV 1

2015-01-31 | E-TABM-1163 | biostudies-arrayexpress

Transcription profiling of human monocyte-derived macrophages infected with HIV-1 or stimulated with interferon-beta, interferon-gamma or lipopolysaccharide

Project description:Transcriptional profiling of HIV-1 infected and that of IFNbeta, IFNgamma or LPS stimulated human monocyte derived macrophages.

2009-09-01 | E-MEXP-2032 | biostudies-arrayexpress

Transcription profiling by array of human and Arabidopsis cells to develop a pipeline for data processing of Agilent oligonucleotide expression arrays

Project description:From analysis of error and reproducibility to a pipeline for data processing of Agilent oligonucleotide expression arrays.

2010-09-07 | E-TABM-942 | biostudies-arrayexpress

Transcription profiling of human monocyte-derived macrophages infected with HIV-1 or uninfected and stimulated with E. coli lipopolysaccharide

Project description:Whole genome expression profiling of HIV-1 infected and uninfected monocyte-derived macrophages

2009-01-01 | E-MEXP-1904 | biostudies-arrayexpress

Transcription profiling by array of pro-inflammatory phenotype of recently isolated human alveolar macrophages

Project description:Human alveolar macrophages and monocyte derived macrophages were compared using genome-wide transcriptional profiling.

2012-09-19 | E-TABM-1206 | biostudies-arrayexpress

Transcriptional response of LPS-stimulated monocyte-derived macrophages in the presence or absence of the anti-TNF agonist Etanercept

Project description:We measured TNFÎ± activity on the transcriptional level by identifying TNFÎ±-inducible genes in human blood monocyte-derived macrophages (MDM). On the basis that TNFÎ± does not act in isolation, we used bacterial lipopolysaccharide (LPS) as a prototypic model of a stimulus to invoke secretion of a wide range of immunologically active factors, including TNFÎ±. In order to identify gene expression attributable to LPS-induced TNFÎ±, we compared the transcriptome of MDM 24 hours after stimulation with 100 ng/ml ultra-pure LPS in the presence and absence of the soluble TNF receptor fusion protein Etanercept (10 Âµg/ml).

2017-08-04 | E-MTAB-5095 | biostudies-arrayexpress

Transcriptional response of monocyte-derived macrophages to stimulation with wildtype or pneumolysin-deficient Streptrococcus pneumoniae in the presence or absence of cytochalasin D

Project description:We used genome-wide transcriptional profiling by microarray to assess the contribution of pneumolysin on macrophage innate immune responses to the TIGR4 strain of Streptococcus pneumoniae (Spn). We focused on the early transcriptional responses at 4 hours after inoculation of human blood monocyte-derived macrophage cultures with Spn at a multiplicity of 10 bacteria to each cell. We compared transcriptomes in the presence and absence of wildtype or pneumolysin-deficient TIGR4 Spn, and also in the presence and absence of cytochalasin D to assess whether there is a differential effect of pneumolysin on innate immune responses with and without bacterial internalisation.

2021-03-31 | E-MTAB-8947 | biostudies-arrayexpress

Transcription profiling of human monocyte-derived macrophages infected in vitro with wild type Leishmania chagasi parasites and then cultured with naive, autologous T-cells

Project description:We performed gene expression analysis using Affymetrix U133Plus2 microarray chips on cultures of infected human cells. To mimic an in vivo infection, we infected human monocyte-derived macrophages (MDMs) in vitro with wild-type L. chagasi parasites and then co-cultured those infected MDMs with naive, autologous T-cells.

2007-11-01 | E-MEXP-1290 | biostudies-arrayexpress

Molecular and functional characterization of alloantigen-specific anergic T-cell suitable for cell therapy

Project description:IL-10 is an immunomodulant cytokine that plays a central role in controlling inflammation, down-regulating immune responses, and inducing immunological tolerance. IL-10 inhibits the expression of costimulatory molecules and the secretion of inflammatory cytokines by antigen-presenting cells (APC), directly suppresses T-cell proliferation, and promotes T-cell anergy. In this study we demonstrate that IL-10 inhibits primary allogeneic proliferation in total PBMC and induces antigen(Ag)-specific T-cell hyporesponsiveness. IL-10-induced anergy is TGF-beta independent, is associated with a decrease of Ag-specific CTLp frequency, and can be obtained among cells with different degree of HLA disparity. The use of tolerogenic dendritic cells (DC) differentiated in the presence of IL-10 (DC-10) allows a more consistent anergy induction, even in the context of HLA matched donors. Importantly, alloAg-IL-10/DC-10-anergized T cells preserve their ability to respond to nominal and third party Ags. Microarray experiments were conducted on different mixed lymphocyte cultures aiming at the identification of gene expression profiles characteristic for the various T cell populations. Independently from the APC used to anergized T cells, the resulting T cell populations exhibit a specific gene signature. Based on the results of this study, we developed a method suitable for GMP scale up and for the generation of a population of anergic Ag-specific T cells for cellular therapy. The dataset comprises 12 samples divided into four sample groups each representing a certain kind of allogeneic mixed lymphocyte culture, i.e. peripheral blood mononuclear cells (PBMC) mixed with CD3-depleted cells (MEC, monocyte-enriched cells) with or without IL-10 treatment, and PBMC mixed with mature or IL-10 treated dendritic cells (DCs). Each group includes three biological replicates with cells collected from different donors.

2010-08-16 | E-GEOD-17493 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data