Transcriptomic and physiological responses to fishmeal substitution with plant proteins in formulated feed in farmed Atlantic salmon (Salmo salar)

ABSTRACT: This experiment was aimed at understanding transcriptional response to a plant protein diet in multiple tissues of Atlantic salmon. Agilent-based microarray platform with 4 x 44 K probes per slide (Salar_2; Agilent Design ID:025520) oligo microarray was used in this experiment. A dual-labelled experimental design was employed for the microarray hybridisations. aRNA from each experimental sample (Cy3 labelled) was competitively hybridised against a common pooled-reference sample (Cy5 labelled), which comprised equal amounts of aRNA from each of the samples used in the study. This design permits valid statistical comparisons across all treatments to be made. The entire experiment comprised 24 hybridisations - 3 tissues (mid intestine, liver, muscle) x 2 treatments (MP diet / PP diet) x 4 bio

ORGANISM(S): Salmo salar

SUBMITTER: Sam Martin 

PROVIDER: E-TABM-1207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress