Transcription profiling of human bronchial epithelial cells from cultures exposed to cigarette smoke or filtered air

ABSTRACT: Cell cultures from three human non-smoker donors were exposed 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke or filtered air for 1h. Cells from four replicate inserts for each dose/time-point were pooled and placed in TRIzol either immediately after exposure or following a 5h or 23h recovery period after the cultures were returned to an incubator at 37ºC, 5% CO2. The ‘no treatment’ control (NTC) remained in the incubator and received neither smoke nor air. In order to perform robust statistical analyses three independent replicate experiments were performed on each of the three donors

ORGANISM(S): Homo sapiens

SUBMITTER: Heather Maunders 

PROVIDER: E-TABM-127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress