RNAi knock down of NIPP1 and EZH2 in human prostate cell line PC-3 to investigate whether they regulate a common set of genes

ABSTRACT: NIPP1 and EZH2 contribute to the silencing of a common set of genes. Since both NIPP1 and EZH2 turned out to be essential for the initiation and maintenance of global H3K27 trimethylation, a key step in the PcG-mediated transcriptional regulation of genes, we have subsequently examined by DNA-microarray analyses whether the loss of NIPP1 or EZH2 results in the expected regulation of a common set of genes.

PC-3 cells were transfected in four independent experiments with a control siRNA or siRNA duplexes for the knockdown of EZH2 or NIPP1. To minimize indirect effects, RNA was already isolated 48h after transfection. At this time, the knockdown of NIPP1 or EZH2 was verified by quantitative qRT-PCR and amounted to 73 ± 7% and 82 ± 3% (n = 4), respectively. The isolated RNA pools we

ORGANISM(S): Homo sapiens

SUBMITTER: Aleyde Van Eynde 

PROVIDER: E-TABM-128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress