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Transcription profiling of human tetraploid and polyploid (8N and 16N) megakaryocytes to identify genes which are up and down regulated during megakaryocyte differentiation/polyploidization

ABSTRACT: The aim of the experiment was to characterize by a pan-genomic approach genes which are continuously up and down regulated during polyploidization during megakaryocyte differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Philippe Dessen

PROVIDER: E-TABM-133 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach.

Raslova Hana H Kauffmann Audrey A Sekkaï Dalila D Ripoche Hugues H Larbret Fréderic F Robert Thomas T Tronik Le Roux Diana D Kroemer Guido G Debili Najet N Dessen Philippe P Lazar Vladimir V Vainchenker William W

Blood 20061214 8

Polyploidization is a part of the normal developmental process leading to platelet production during megakaryocyte (MK) differentiation. Ploidization is mainly involved in cell enlargement, but it is not clear whether gene expression is modified during MK ploidization. In this study, human MKs were grown from CD34(+) cells in the presence of thrombopoietin and sorted according to their ploidy level. A pangenomic microarray technique was applied to compare gene expression in 2N-, 4N-, 8N-, and 16 ...[more]

PMID: 17170127

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