Chromatin immunoprecipitation of Twist protein during embryonic development in Drosophila

ABSTRACT: Genomewide mapping of Drosophila Twist protein binding during embryonic development. Two consecutive timepoints (2-4 and 4-6 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Twist protein. Additionally, preimmune-serum was used as a control. The enriched DNA was hybridized to custom designed tiling arrays optimized for assaying all E-box motifs outside repetitive or coding regions of the Drosophila melanogaster genome (average gap size = 290 bps).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot 

PROVIDER: E-TABM-159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress