Transcription profiling of eight different time periods of the Anopheles gambiae lifecycle

ABSTRACT: We conducted a genome wide survey of mosquito gene expression profiles during 8 different time periods of the Anopheles gambiae lifecycle. The timeperiods included embryos, E, collected 12-14 hours post oviposition (hpo), five larval samples, La-Le, with La collected approximately 48h hpo and the rest, Lb-Le, collected at 48 intervals thereafter, irrespective of larval size and instar, pupae, P, collected at the same time as Le samples (240 hpo) and freshly emerged adult females, F and males, M, collected 312 hpo. To investigate mosquito expression during development, we performed competitive two-dye hybridizations of experimental and standard reference RNA samples to MMC1 microarrays. MMC1 microarrays contain approximately 20,000 EST clones. Standard reference RNA was produced in vitro fr

ORGANISM(S): Anopheles gambiae

SUBMITTER: unknown MacCallum 

PROVIDER: E-TABM-186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress