Poly(A) tail length profiling of mRNAs from fission yeast

ABSTRACT: To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarrays using total eluate as a reference.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Daniel Lackner 

PROVIDER: E-TABM-217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress