Transcription profiling of human umbilical vein endothelial cells (HUVECs) transduced with pMLV-MCS or pMLV-THAP1 retroviral expression vectors

ABSTRACT: Goal of the experiment : Retroviral-mediated gene transfer of the THAP-zinc finger protein THAP1 inhibits endothelial cell proliferation through coordinated repression of critical cell cycle regulators and pRB-E2F target genes. Experimental design: To gain insight into the effects of THAP1 on endothelial cell growth regulatory pathways, we identified THAP1 target genes in primary human endothelial cells using oligonucleotide-based microarray technology. Human umbilical vein endothelial cells (HUVECs) were transduced with pMLV-MCS or pMLV-THAP1 retroviral expression vectors and, after 48h, cells were harvested for isolation of total RNA and preparation of Cy3- or Cy5-labeled cRNA probes, which were hybridized to DNA microarrays that contained 22 000 unique 60-nt oligonucleotide probes rep

ORGANISM(S): Homo sapiens

SUBMITTER: François AMALRIC 

PROVIDER: E-TABM-24 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress