Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of yeast temperature-sensitive abf1-1 cells when grown at the restrictive temperature of 37oC as compared to wild-type cells grown under same conditions


ABSTRACT: The aim of this study is to define the groups of genes which are de-regulated in temperature-sensitive abf1-1 cells when grown at the restricitve temperature of 37 degrees C as compared to wild-type cells grown under same conditions. Control samples also include RNA extracted from mutant and wild-type cells grown at the permissive temperature of 25°C. Furthermore, de-regulation was measured not only under mitotic growth conditions (YPD and YPA) but also at two time-points during sporulation (at 6h and 10h).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: James Moore 

PROVIDER: E-TABM-291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast.

Schlecht Ulrich U   Erb Ionas I   Demougin Philippe P   Robine Nicolas N   Borde Valérie V   van Nimwegen Erik E   Nicolas Alain A   Primig Michael M  

Molecular biology of the cell 20080227 5


The autonomously replicating sequence binding factor 1 (Abf1) was initially identified as an essential DNA replication factor and later shown to be a component of the regulatory network controlling mitotic and meiotic cell cycle progression in budding yeast. The protein is thought to exert its functions via specific interaction with its target site as part of distinct protein complexes, but its roles during mitotic growth and meiotic development are only partially understood. Here, we report a c  ...[more]

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