Comparative genomic hybridization of human chronic lymphocytic leukemia samples with a small 13q14 deletion and normal genomic DNA.

ABSTRACT: It has been widely recognized that a 13q14 deletion is often found in CLL and that this deletion is associated with a good prognosis. Such deletions are sometime detected only by fluorescence in situ hybridization (FISH) [1] because they are too small to be detected by banding. We report here a small deletion that is undetectable by FISH but identifiable by high-resolution comparative genomic hybridization (CGH) arrays. A 62-year-old patient with previously untreated chronic lymphocytic leukemia (CLL; BinetÕs stage A) was studied because of a recent increase of his lymphocyte counts. The patient had been in stage A since the initial diagnosis was made 12 years before. At the time of cytogenetic analysis, the peripheral lymphocyte count was 65.3 g/L with weak llight chain surface imunoglobulin expression and a Matutes score of 5/5. The karyotype of 12-O-tetradecanoylphorbal 13-acetate-stimulated blood cells was 46,X,-Y[3]/46,XY [17]. FISH with X and Y probes confirmed that 90% of the blood cells had a Y chromosome loss, which is an infrequent but recurrent finding in CLL. FISH of 200 cells found no 13q14 deletion.

ORGANISM(S): Homo sapiens

SUBMITTER: Philippe Dessen 

PROVIDER: E-TABM-293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress