Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling time series of rep1delta mutant fission yeast during meiosis

ABSTRACT: Changes in gene expression are hallmarks of cellular differentiation. Sexual differentiation in fission yeast (Schizosaccharomyces pombe) provides a model system for gene expression programs accompanying and driving cellular specialization. The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes. We used DNA microarrays to investigate meiotic gene regulation by examining transcriptomes after genetic perturbations (gene deletion and/or overexpression) of rep1, mei4, atf21 and atf31, which encode known transcription factors controlling sexual differentiation. This analysis reveals target genes at a genome-wide scale and uncovers combinatorial control by Atf21p and Atf31p. We also studied two transcription factors that are upregulated during meiosis but had not been previously implicated in sexual differentiation : Rsv2p induces stress-related genes during spore formation, while Rsv1p acts as a repressor for glucose-metabolism genes. Our data further reveal negative feedback interactions: both Rep1p and Mei4p not only activate specific gene expression waves (early and middle genes, respectively), but are also required for repression of genes induced in the previous waves (Ste11p-dependent and early genes, respectively).

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata

PROVIDER: E-TABM-300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling time series of mei4delta mutant fission yeast during meiosis

Project description:Changes in gene expression are hallmarks of cellular differentiation. Sexual differentiation in fission yeast (Schizosaccharomyces pombe) provides a model system for gene expression programs accompanying and driving cellular specialization. The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes. We used DNA microarrays to investigate meiotic gene regulation by examining transcriptomes after genetic perturbations (gene deletion and/or overexpression) of rep1, mei4, atf21 and atf31, which encode known transcription factors controlling sexual differentiation. This analysis reveals target genes at a genome-wide scale and uncovers combinatorial control by Atf21p and Atf31p. We also studied two transcription factors that are upregulated during meiosis but had not been previously implicated in sexual differentiation : Rsv2p induces stress-related genes during spore formation, while Rsv1p acts as a repressor for glucose-metabolism genes. Our data further reveal negative feedback interactions: both Rep1p and Mei4p not only activate specific gene expression waves (early and middle genes, respectively), but are also required for repression of genes induced in the previous waves (Ste11p-dependent and early genes, respectively).

2008-01-01 | E-TABM-298 | biostudies-arrayexpress

Transcription profiling of wild type, rsv1 deletion and SPBC11-5.14 deletion mutant fission yeast

2008-01-01 | E-TABM-301 | biostudies-arrayexpress

Transcription profiling of S. pombe wild type and meu5 deletion mutants during meiosis

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. To investigate the function of Meu5 we induced synchronous meiosis in wild type or meu5delta cells (using pat1 thermo-sensitive mutations), and measured mRNA levels at regular intervals.

2011-01-01 | E-TABM-774 | biostudies-arrayexpress

Transcription profiling of S. pombe wild type and meu5delta cells during pat1 induced meiosis, wild type meiosis and overexpression of the mei4 transcription factor

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. We compared wild type and meu5delta cells transcriptome under different conditions: pat1-induced meiosis in diploid cells, wild type meiosis in diploid cells and cells overexpressing the Mei4 transcription factor.

2011-01-01 | E-TABM-775 | biostudies-arrayexpress

Transcription profiling of fission yeast cell cycle, elutriation 2

Project description:Cell cycle timecourse of fission yeast using a selective synchronisation method ? elutriation nr. 2

2004-04-01 | E-MEXP-55 | biostudies-arrayexpress

Transcription profiling of fission yeast cell cycle, elutriation 1

Project description:Cell cycle timecourse of fission yeast using a selective synchronisation method elutriation nr. 1

2004-04-01 | E-MEXP-56 | biostudies-arrayexpress

Transcription profiling of fission yeast mutants sep10 and sep15

Project description:The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression with cell division. Mutants in mcs6 and pmh1 arrest with incomplete cell separation and decreased phosphorylation of the Pol II large subunit. Gene expression profiling by microarray hybridization revealed that a defined subset (~5%) of genes was repressed by Mcs6 complex impairment, whereas the majority was refractory. The repression signature included a cell-cycle cluster implicated in cytokinesis and cell separation, and overlapped with those of the cell-separation mutants sep1, sep10 and sep15 (relative to wild-type cells). The gene sep10 encodes the homolog of a protein found in metazoan Mediator-like complexes, and sep15 encodes an established component of the Mediator. In mcs6 or pmh1 mutants, sep10+, which also encodes a component of the transcriptional machinery, becomes essential for viability. Finally, mcs6+ also interacts genetically with sep1+, which encodes a forkhead transcription factor required for periodic transcription during mitosis and cell division. Thus, the Mcs6 complex (a direct activator of the cell-cycle engine) also helps govern the cell-cycle transcriptional program.

2004-11-01 | E-MEXP-193 | biostudies-arrayexpress

Transcription profiling of S. pombe meu5delta and wild type cells over-expressing the transcription factor mei4 to determine mRNA half-lives

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of meu5delta and wild type cells over-expressing the transcription factor Mei4.

2011-01-01 | E-TABM-776 | biostudies-arrayexpress

Chromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast

Project description:The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays

2007-03-22 | E-TABM-219 | biostudies-arrayexpress

Transcription profiling of fission yeast cell cycle, cell cycle overexpressions

Project description:Cell cycle regulation in fission yeast using overexpression of transcription factors

2004-04-01 | E-MEXP-63 | biostudies-arrayexpress