Transcription profiling of Mycobacterium tuberculosis wild type, ideR mutant and ideR complemented mutants strains grown in iron-sufficient or iron-deficient media

ABSTRACT: The mycobacterial IdeR protein is a metal-dependent regulator of the DtxR (diphtheria toxin repressor) family. In the presence of iron, it binds to a specific DNA sequence in the promoter regions of the genes that it regulates, thus controlling their transcription. In this study, we provide evidence that ideR is an essential gene in Mycobacterium tuberculosis. ideR cannot normally be disrupted in this mycobacterium in the absence of a second functional copy of the gene. However, a rare ideR mutant was obtained in which the lethal effects of ideR inactivation were alleviated by a second-site suppressor mutation and which exhibited restricted iron assimilation capacity. Studies of this strain and a derivative in which IdeR expression was restored allowed us to identify phenotypic effects res

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Jeannette Barba 

PROVIDER: E-TABM-317 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress