RNAi knock down of PLC gamma 2, CaMKII, PKC delta and Pyk2 in mouse B cell line A20 after stimulation by anti-IgG to examine the adaptation response of the BCR-dependent intracellular signaling network

ABSTRACT: We examined the adaptation response of the BCR-dependent intracellular signaling network, to targeted perturbations induced through siRNA-mediated depletion of select signaling intermediates. The robustness of this network was found to derive from the fact that its constituent nodes displayed graded sensitivities to these perturbations. Importantly, this latter property also facilitated generation of perturbation-unique calibration of signal at individual nodes, with a corresponding effect on the cellular phenotypic response. Subsequently generated data-driven models revealed that the information specifying cellular phenotypic responses was not coded within variations of groups of nodes acting in a discrete manner. Rather, characteristic signaling features were selectively extracted from the majority of the constituent nodes, and then integrated to define a response-specific axis. Thus, stimulus-induced cell fate decisions are governed through a mechanism that defines the composition of signal extracted, from the overall noise provided by the assortment of signals generated at each of the nodes. The microarray experiment was performed here to identify differences in cellular responses upon various cellular perturbations in terms of gene expression profile. Cells were transfected with specific siRNAs against PLC gamma 2, CaMKII, PKC delta and Pyk2 in addition to mock siRNAs.

ORGANISM(S): Mus musculus

SUBMITTER: Helena Ahlfors 

PROVIDER: E-TABM-360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress