RNAi knock down of PLC gamma 2, CaMKII, PKC delta and Pyk2 in mouse B cell line A20 after stimulation by anti-IgG to examine the adaptation response of the BCR-dependent intracellular signaling network

ABSTRACT: We examined the adaptation response of the BCR-dependent intracellular signaling network, to targeted perturbations induced through siRNA-mediated depletion of select signaling intermediates. The robustness of this network was found to derive from the fact that its constituent nodes displayed graded sensitivities to these perturbations. Importantly, this latter property also facilitated generation of perturbation-unique calibration of signal at individual nodes, with a corresponding effect on the cellular phenotypic response. Subsequently generated data-driven models revealed that the information specifying cellular phenotypic responses was not coded within variations of groups of nodes acting in a discrete manner. Rather, characteristic signaling features were selectively extracted from t

ORGANISM(S): Mus musculus

SUBMITTER: Helena Ahlfors 

PROVIDER: E-TABM-360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress