Transcription profiling of human CD4+ leukemia Jurkat T-cells in which the PI3K pathway is constitutively turned-on and FOXO1 transcriptional activity strongly repressed

ABSTRACT: One major effect of PI3-kinase activation downstream of the serine/threonine kinase Akt is the phosphorylation of the transcription factor FOXO1 and its neutralization. FOXO1 has several ubiquitous targets genes in many cell types that control cell quiescence, oxydative stress or apoptosis. However, it has been demonstrated that FOXO1 also has specific targets depending of the cellular context. The role of FOXO1 to regulate specific genes in T lymphocytes has not been investigated yet. To examine this point, we used the CD4+ leukemia Jurkat T-cell line, in which the PI3K pathway is constitutively turned-on and FOXO1 transcriptional activity strongly repressed. These cells were transduced with lentiviruses coding for a constitutively active form of FOXO1 fused to GFP and having the three AK

ORGANISM(S): Homo sapiens

SUBMITTER: Hugues Ripoche 

PROVIDER: E-TABM-391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress