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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat pancreatic INS-1E cells submitted to SILAC and different glucose levels

ABSTRACT: mRNAs of INS-1E cells submitted to SILAC and different glucose levels were compared

ORGANISM(S): Rattus norvegicus

SUBMITTER: Yohann Coutï¿½

PROVIDER: E-TABM-477 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Interactome of Ca2+-bound secretagogin in INS-1E cells

Project description:By using His6-tagged recombinant human secretagogin in the presence of Ca2+ (100 µM), we precipitated putative interacting partners from INS-1E cells and identified their amino acid sequences by mass spectrometry. We detected canonical interacting proteins participating in vesicle-mediated transport, exocytosis and cytoskeletal organization. Besides, we captured proteins controlling protein folding and (de-)ubiquitination. Our data support that secretagogin modulates protein folding and degradation through protein-protein interactions and interacts with USP9X in β cells to control cell survival.

2017-06-21 | PXD006544 | Pride

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Project description:This study aims at elucidating how Coxsackie B virus infection perturbs the host's miRNA regulatory pathways that may lead to different pathological events using the miRNA microarray approach. The rat pancreatic cell line - INS-1E, was infected with various preparations of Coxsackie B4 viruses was analysed for miRNA expression profiles subsequently. The miRNA expression profiles were measured at 48, and 72 hours post infection, respectively.

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Expression data from rat INS-1E cells

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Project description: Not available

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