Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

25 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of hepatocytes from mice that are hyperglycaemic, obese and insulin resistant as a result of being fed a high fat diet - Illumina dataset

ABSTRACT: Effect of high fat diet feeding on gene expression

ORGANISM(S): Mus musculus

DISEASE(S): hyperglycaemic

SUBMITTER: Dominique Gauguier Steven Wilder

PROVIDER: E-TABM-548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of rat kidney and liver from several different strains to determine the effects of hyperglycaemia and genetic background difference on gene expression

Project description:Effects of hyperglycaemia and genetic background differences on renal and hepatic gene expression

2009-06-03 | E-TABM-502 | biostudies-arrayexpress

Eliane_IL2.Rapa bis

Project description:Administration of low-dose IL-2 prevents and cures type 1 diabetes (T1D) in NOD mice by boosting pancreatic regulatory T cells (Tregs) and is currently being evaluated in humans. Here, to improve treatment efficacy we tested higher IL-2 doses that, despite further boosting Tregs, rapidly precipitated T1D in pre-diabetic mice due to generalized immune activation. Although the combination of rapamycin (RAPA) plus IL-2 prevents NOD T1D development, a recent clinical trial translating this strategy into patients was halted due to C-peptide decline. Here, we show that RAPA/IL-2 combination was also ineffective to cure new onset T1D in mice and, surprisingly, RAPA broke IL-2-induced tolerance in a reversible way. RAPA partially counteracted IL-2 effects on Tregs and the combined treatment unexpectedly, impaired glucose homeostasis at multiple levels, possibly explaining the clinical outcome. Our data help understand IL-2 alone or RAPA/IL-2 combination limitations and could lead to the design of improved T1D therapies.

2013-12-10 | E-MEXP-3789 | biostudies-arrayexpress

Foie WTS vs WT

Project description:Transcriptional and polysomal analysis of mice liver hepatocytes after 4-hour refeeding as compared to overnight starved controls.

2013-01-24 | E-MEXP-3522 | biostudies-arrayexpress

siRNA profiling of human synovial fibroblasts depleted for clusterin

Project description:Analysis of synoviocytes cell form patients suffering of Rheumatoid arthritis and depleted for clusterin by siRNA knockdown. Note: this experiment was reloaded into ArrayExpress on 2nd December 2009 because the incorrect array design had originally been selected. The identifiers in the datafile were also fixed to match the array design.

2009-12-04 | E-TABM-653 | biostudies-arrayexpress

OJeanJean_eRF3a_ATF4

Project description:We previously showed that eRF3a depletion in the human colon carcinoma cell line HCT116 p53+/+ induces mTOR signaling pathway inhibition (Chauvin et al., 2007) suggesting that eRF3a belongs to a regulatory mechanism that modulate mTOR activity. However, this mechanism and the precise role of eRF3a remain to be determined. Here, to elucidate the mechanism dictating mTOR signaling pathway inhibition, we aimed to define the transcript expression and polysome profiles of HCT116 cells after short interfering RNA (siRNA)-mediated depletion of eRF3a. Three days after HCT116 cells electroporation with either sh-3a1 which was previously shown for effectively silence eRF3a (Chauvin et al., 2005) or control shRNA (sh-Ctrl), total and polysome-associated RNAs were extracted and we conducted a microarray analysis of differentially expressed genes using the Illumina HumanHT-12 Expression BeadChips.

2012-08-30 | E-MEXP-3512 | biostudies-arrayexpress

Transcription profiling by array of mouse CD4+ GFP+ (tregs) and CD4+ GFP- (Teffs) cells treated with IL-2

Project description:To obtain the molecular signature of the effect that the IL-2 therapeutic dose induces on T cells in vivo, we performed gene array analysis. Since it was critical to perform this analysis on highly purified Tregs and Teffs, and also because activated Teffs can acquire CD25 expression, we used GFP-Foxp3 knock-in mice which allow for the purification of Tregs and Teffs based on the GFP expression. Illumina gene array was carried out on FACS sorted CD4+ GFP+ (Tregs) and CD4+ GFP- (Teffs) of mice that received either PBS or the curative schedule of IL-2, 2 hours after the last injection.

2011-03-21 | E-MEXP-2689 | biostudies-arrayexpress

Transcription profiling of human brain tissue from an individual with neurological disease

Project description:Transcriptomic comparison of brain tissues

2010-11-25 | E-TABM-888 | biostudies-arrayexpress

Transcription profiling of mouse CD4+ GFP- (Teff) and CD4+ GFP+ (Treg) T cells treated with IL-2

2011-01-06 | E-MEXP-2530 | biostudies-arrayexpress

S6K_RiBi

Project description:Transcriptional analysis of concequent lack of rpS6 kinase activity in mice livers hepatocytes due to deletion of S6 kinases 1 and 2 (S6K1 and S6K2).

2013-01-16 | E-MEXP-3518 | biostudies-arrayexpress

Transcription profiling of human breast cell lines and one normal RNA sample

Project description:Total RNA from seven breast cell lines and one normal RNA samples was labelled using the Illumina TotalPrep RNA Amplification kit (Ambion) following manufacturer's instructions. 1.5 ug of biotin-labelled cRNA were used for each hybridisation on Sentrix Human-6 BeadChips (Illumina, San Diego, CA) following manufacturer's protocol.

2007-11-17 | E-TABM-194 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data