Chromatin immunoprecipitation of Mef2 protein in Drosophila during embryonic development

ABSTRACT: Genomewide mapping of Drosophila Mef2 protein binding during embryonic development. Five consecutive timepoints (2-4, 4-6, 6-8, 8-10, 10-12 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Mef2 protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot 

PROVIDER: E-TABM-56 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress