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Transcription profiling of chicken lung to investigate responses to avian influenza virus

ABSTRACT: Transcription profiling of chicken lung to investigate responses to avian influenza virus

ORGANISM(S): Gallus gallus

SUBMITTER: Sander van Hooff

PROVIDER: E-TABM-636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow.

Reemers Sylvia S SS van Haarlem Daphne A DA Groot Koerkamp Marian J MJ Vervelde Lonneke L

The Journal of general virology 20090603 Pt 9

Sampling the complete organ instead of defined parts might affect analysis at both the cellular and transcriptional levels. We defined host responses to H9N2 avian influenza virus (AIV) in trachea and different parts of the lung. Chickens were spray-inoculated with either saline or H9N2 AIV. Trachea and lung were sampled at 1 and 3 days post-inoculation (p.i.) for immunocytochemistry, real-time quantitative RT-PCR and gene-expression profiling. The trachea was divided into upper and lower parts ...[more]

PMID: 19494054

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