Transcription profiling of mouse embyronic stem cells cultured with PI3-K signalling inhibitor LY294002 to identify PI3K-target genes

ABSTRACT: This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, http://www.fungenes.org).The experiment was conducted at the Unyversity of Bath, Bath, UK. Aim of the experiment is the identification of genes regulated by PI3K-dependent signaling in undifferentiated ES cells. Materials and methods: To identify target genes of PI3-K signalling, the PI3-K signalling inhibitor LY294002 was used at a concentration which was shown to perturb self-renewal in the presence of LIF and which knocks down PI3K signals. CGR8 cells cultivated in serum-free conditions in the presence of LIF were starved for 24h in media lacking LIF and then pre-inubated for 30 minutes with 5 mM LY294002 in DMSO o

ORGANISM(S): Mus musculus

SUBMITTER: Oliver Hummel 

PROVIDER: E-TABM-673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress