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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human cell lines treated with cytochalasin D and nocodazole with the aim of characterising tetraploid clones

ABSTRACT: During the oncogenic process, tetraploidy is a candidate intermediate stage leading from diploidy to aneuploidy. The aim of our experiment was to establish tetraploid clones and to characterize their transcriptome.

ORGANISM(S): Homo sapiens

SUBMITTER: Nazanine Modjtahedi

PROVIDER: E-TABM-70 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Apoptosis regulation in tetraploid cancer cells.

Castedo Maria M Coquelle Arnaud A Vivet Sonia S Vitale Ilio I Kauffmann Audrey A Dessen Philippe P Pequignot Marie O MO Casares Noelia N Valent Alexandre A Mouhamad Shahul S Schmitt Elise E Modjtahedi Nazanine N Vainchenker William W Zitvogel Laurence L Lazar Vladimir V Garrido Carmen C Kroemer Guido G

The EMBO journal 20060504 11

Tetraploidy can result in cancer-associated aneuploidy. As shown here, freshly generated tetraploid cells arising due to mitotic slippage or failed cytokinesis are prone to undergo Bax-dependent mitochondrial membrane permeabilization and subsequent apoptosis. Knockout of Bax or overexpression of Bcl-2 facilitated the survival of tetraploid cells at least as efficiently as the p53 or p21 knockout. When tetraploid cells were derived from diploid p53 and Bax-proficient precursors, such cells exhib ...[more]

PMID: 16675948

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Transcription profiling of non-small cell lung carcinoma cell line H1975 treated with cisplatin or BMS-69014 a novel panHER/VEGFR inhibitor

Project description:Non-small cell lung cancer (NSCLC) with activating mutations in the epidermal growth factor receptor (EGFR) responds to EGFR tyrosine kinase inhibitors such as erlotinib. However, secondary somatic EGFR mutations (e.g. T790M) confer resistance to erlotinib. BMS-690514, a novel panHER/VEGFR inhibitor described here, exerted antiproliferative and pro-apoptotic effects on NSCLC cell lines, with prominent efficacy on H1975 cells expressing the T790M mutation. In this model, BMS-690514 induced a G1 cell cycle arrest, as well as ultrastructural hallmarks of apoptosis, mitochondrial release of cytochrome c, and activation of caspases involved in the intrinsic (e.g. caspase -2, -3, -7 and -9), but not in the extrinsic (e.g. caspase-8) pathway. Caspase inhibition conferred partial protection against BMS-690514 cytotoxicity, pointing to the involvement of both caspase-dependent and -independent effector mechanisms. Transcriptome analyses revealed the upregulation of pro-apoptotic (e.g. Bim, Puma) and cell cycle inhibitory (e.g. p27Kip1, p57Kip2) factors, as well as the downregulation of anti-apoptotic (e.g. Mcl1), heat shock (e.g. HSP40, HSP70, HSP90) and cell cycle promoting (e.g. cyclins B1, D1 and D3, CDK1, MCM family proteins, PCNA) proteins. BMS-690514-induced death of H1975 cells was modified in a unique fashion by a panel of siRNAs targeting apoptosis modulators. Downregulation of components of the NF-kappaB survival pathway (e.g. p65, Nemo/IKK, TAB2) sensitized cells to BMS-690514, whereas knockdown of pro-apoptotic factors (e.g. Puma, Bax, Bak, caspase-2, etc) and DNA damage-related proteins (e.g. ERCC1, hTERT) exerted cytoprotective effects. BMS-690514 is a new pan-HER/VEGFR inhibitor that may become an alternative to erlotinib for the treatment of NSCLC.

2007-07-24 | E-TABM-239 | biostudies-arrayexpress

Transcription profiling of human T cell lines isolated from tumor infiltrating lymphocytes or peripheral blood lymphocytes of a patient with large cell carcinoma of the lung

Project description:The IGR-Heu tumor cell line was established from patient Heu suffering from large cell carcinoma of the lung. Heu171 T cell clones were isolated from autologous tumor infiltrating lymphocytes. H32-22 clone was isolated from autologous peripheral blood lymphocytes after stimulation with IGR-Heu and sorting with peptide-MHC tetramers. T cells were grown in the presence of irradiated autologous tumor and lymphoblastoid cell lines in complete media supplemented with rIL-2 and conditioned medium from phytohemagglutinin-activated lymphocytes for 3 weeks. Then, for microarray assays, RNA was extracted from the different cells

2007-03-02 | E-TABM-207 | biostudies-arrayexpress

Transcription profiling of response to tamoxifen in human ERa-positive postmenopausal breast tumor patients

Project description:Identification of HRPAP20 and TIMELESS as outstanding candidate markers to predict the response to tamoxifen in ERa-positive postmenopausal breast tumor patients

2007-10-09 | E-TABM-72 | biostudies-arrayexpress

Transcription profiling of 9 pairs of human metastasis/primary melanoma (each from the same patient)

Project description:Study by dye-swap of 9 pairs of metastasis/primary melanoma (each from the same patient)

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Transcription profiling of cohort of human cutaneous primary melanomas associated with a distant metastasis free survival

Project description:Identification of genomic characteristics in a cohorte of human cutaneous primary melanoma associated with a distant metastasis free survival.

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Comparative genomic hybridization of 23 human nevi with a pool of primary cutaneous melanoma

Project description:Genomic profiles comparing 23 nevi with a pool of primary cutaneous melanoma

2009-04-16 | E-TABM-3 | biostudies-arrayexpress

Transcription profiling of human cutaneous primary melanomas in an independent population of 17 patients

Project description:Validation of genomics characteristics of human cutaneous primary melanoma in an independnat population of 17 patients.

2007-04-01 | E-TABM-2 | biostudies-arrayexpress

Transcription profiling of human diploid and tetraploid HCT116 cells treated with cisplatin and/or a Chk1 inhibitor to investigate resistance to genotoxic stress

Project description:Tetraploid cells are more resistant against genotoxic stress (irradiation, platinum compounds, topoisomerase inhibitors) than their diploid precursors. Here, we studied the effect of cisplatin and/or Chk1 inhibition on the transcriptome of diploid and tetraploid HCT116 cells

2008-06-09 | E-TABM-172 | biostudies-arrayexpress

Transcription profiling of humoral resistance to the CTL after phenotypical and functional study of the resistant clone IGR-HeuR8

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2006-01-23 | E-TABM-71 | biostudies-arrayexpress

Transcription profiling of human CD34+ cells from familial platelet disorder

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2010-09-11 | E-TABM-792 | biostudies-arrayexpress

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