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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat heart from Lewis strain immunized with a peptide of human alpha1A-adrenergic receptor

ABSTRACT: Gene expression profiles in hearts of Lewis rats immunized with a peptide of human alpha1A-adrenergic receptor. The pathophysiological relevance of chronic autoantibodies against alpha-1A-adrenergic receptor stimulation in rats was investigated. Lewis rats were immunized using synthesized peptides of second extracellular loop of the human alpha-1A-adrenergic receptor and raised for one year. The gene expression in hearts of three immunized and three control rats were analyzed using Affymetrix Rat Genome 230 2.0 Arrays.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Oliver Hummel

PROVIDER: E-TABM-725 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alpha1A-adrenergic receptor-directed autoimmunity induces left ventricular damage and diastolic dysfunction in rats.

Wenzel Katrin K Wallukat Gerd G Qadri Fatimunnisa F Hubner Norbert N Schulz Herbert H Hummel Oliver O Herse Florian F Heuser Arnd A Fischer Robert R Heidecke Harald H Luft Friedrich C FC Muller Dominik N DN Dietz Rainer R Dechend Ralf R

PloS one 20100224 2

<h4>Background</h4>Agonistic autoantibodies to the alpha(1)-adrenergic receptor occur in nearly half of patients with refractory hypertension; however, their relevance is uncertain.<h4>Methods/principal findings</h4>We immunized Lewis rats with the second extracellular-loop peptides of the human alpha(1A)-adrenergic receptor and maintained them for one year. Alpha(1A)-adrenergic antibodies (alpha(1A)-AR-AB) were monitored with a neonatal cardiomyocyte contraction assay by ELISA, and by ERK1/2 ph ...[more]

PMID: 20195525

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