Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human amniotic fluid after `amnion treatment with surfactant protein-A

ABSTRACT: We investigated a potential role for SP-A in human pregnancy and parturition by examining SP-A expression patterns in amniotic fluid and the amnion

ORGANISM(S): Homo sapiens

SUBMITTER: Adi Tarca

PROVIDER: E-TABM-741 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Surfactant protein-A as an anti-inflammatory component in the amnion: implications for human pregnancy.

Lee Deug-Chan DC Romero Roberto R Kim Chong Jai CJ Chaiworapongsa Tinnakorn T Tarca Adi L AL Lee JoonHo J Suh Yeon-Lim YL Mazaki-Tovi Shali S Vaisbuch Edi E Mittal Pooja P Draghici Sorin S Erez Offer O Kusanovic Juan Pedro JP Hassan Sonia S SS Kim Jung-Sun JS

Journal of immunology (Baltimore, Md. : 1950) 20100503 11

The mechanism of mouse parturition is thought to involve myometrial infiltration by amniotic fluid (AF) macrophages, activated by surfactant protein-A (SP-A). In humans, the concentration of AF SP-A decreases during labor, and no fetal macrophages are found in the myometrium after labor. Therefore, it appears that the mechanisms of labor in mice and humans are different. We investigated a potential role for SP-A in human pregnancy and parturition by examining SP-A expression patterns in AF and a ...[more]

PMID: 20439915

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Project description:Intra-amniotic infection, the invasion of microbes into the amniotic cavity resulting in an inflammatory process, is a clinical condition that can lead to adverse pregnancy outcomes for the mother and fetus as well as severe long-term neonatal morbidities. Despite much research focused on the consequences of intra-amniotic infection, there is still little knowledge about the functional roles of innate immune cells that respond to invading microbes. In the current study, we performed RNA sequencing of sorted neutrophils and monocytes/macrophages from amniotic fluid from women with intra-amniotic infection to determine the transcriptomic differences between these innate immune cells. Further, we sought to identify specific transcriptomic pathways that were significantly altered by the maternal or fetal origin of amniotic fluid neutrophils and monocytes, the presence of a severe fetal inflammatory response, and pregnancy outcome (i.e. preterm or term delivery). We showed that significant transcriptomic differences exist between amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection that are indicative of the distinct roles these cells play. We also found that amniotic fluid monocytes/macrophages of fetal origin display impaired ability to clear out microbes invading the amniotic cavity compared to those of maternal origin. Notably, we demonstrate that the transcriptomic changes in amniotic fluid monocytes/macrophages are heavily associated with the severity of the fetal inflammatory response, suggesting that the trafficking of fetal neutrophils throughout the umbilical cord is partially modulated by monocytes/macrophages in the amniotic cavity. Finally, we show that amniotic fluid neutrophils and monocytes/macrophages from preterm deliveries display enhanced transcriptomic activity compared to those from term deliveries, highlighting the protective role of these innate immune cells in this vulnerable period. Collectively, these findings demonstrate the underlying complexity of local innate immune responses in women with intra-amniotic infection, and provide new insights into the functions of amniotic fluid neutrophils and monocytes in the amniotic cavity.

Dataset Information

Transcription profiling of human amniotic fluid after `amnion treatment with surfactant protein-A

Publications

Surfactant protein-A as an anti-inflammatory component in the amnion: implications for human pregnancy.

Similar Datasets

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