Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

17 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of S. pombe wild type and meu5delta cells during pat1 induced meiosis, wild type meiosis and overexpression of the mei4 transcription factor

ABSTRACT: The Meu5 RNA-binding protein stabilises its targets during meiosis. We compared wild type and meu5delta cells transcriptome under different conditions: pat1-induced meiosis in diploid cells, wild type meiosis in diploid cells and cells overexpressing the Mei4 transcription factor.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata

PROVIDER: E-TABM-775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of S. pombe wild type and meu5 deletion mutants during meiosis

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. To investigate the function of Meu5 we induced synchronous meiosis in wild type or meu5delta cells (using pat1 thermo-sensitive mutations), and measured mRNA levels at regular intervals.

2011-01-01 | E-TABM-774 | biostudies-arrayexpress

Transcription profiling of S. pombe meu5delta and wild type cells over-expressing the transcription factor mei4 to determine mRNA half-lives

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of meu5delta and wild type cells over-expressing the transcription factor Mei4.

2011-01-01 | E-TABM-776 | biostudies-arrayexpress

Transcription profiling of fission yeast mutants sep10 and sep15

Project description:The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression with cell division. Mutants in mcs6 and pmh1 arrest with incomplete cell separation and decreased phosphorylation of the Pol II large subunit. Gene expression profiling by microarray hybridization revealed that a defined subset (~5%) of genes was repressed by Mcs6 complex impairment, whereas the majority was refractory. The repression signature included a cell-cycle cluster implicated in cytokinesis and cell separation, and overlapped with those of the cell-separation mutants sep1, sep10 and sep15 (relative to wild-type cells). The gene sep10 encodes the homolog of a protein found in metazoan Mediator-like complexes, and sep15 encodes an established component of the Mediator. In mcs6 or pmh1 mutants, sep10+, which also encodes a component of the transcriptional machinery, becomes essential for viability. Finally, mcs6+ also interacts genetically with sep1+, which encodes a forkhead transcription factor required for periodic transcription during mitosis and cell division. Thus, the Mcs6 complex (a direct activator of the cell-cycle engine) also helps govern the cell-cycle transcriptional program.

2004-11-01 | E-MEXP-193 | biostudies-arrayexpress

Transcription profiling of fission yeast with deletion or overexpression of ste11 and changes in gene expression after nitrogen starvation of h plus, h minus, h90fus1 and h90ste11 cells to investigate control of gene expression during early sexual differentiation

Project description:Fission yeast cells belong to one of two specialized cell types, M or P. Specific environmental conditions trigger sexual differentiation, which leads to an internal program starting with pheromone signalling between M and P cells, followed by mating, meiosis and sporulation. The initial steps of this process are controlled by Ste11p, a master transcriptional regulator that activates the expression of cell type-specific genes (only expressed in either M or P cells) as well as genes expressed in both M and P cells. <br><br> Pheromone signalling is activated by Ste11p-dependent transcription, and in turn enhances some of this transcription in a positive feedback. To obtain a genome-wide view of Ste11p target genes, their cell-type specificity, and their dependence on pheromone, we used DNA microarrays along with different genetic and environmental manipulations of fission yeast cells.We directly compared the transcriptome of homothallic wild-type cells (h90 fus1) with that of ste11 delta mutants under conditions that induce sexual differentiation. To allow for indirect effects of the ste11 delta mutation, we took advantage of the fact that ectopic expression of ste11 can drive cells into sexual differentiation and therefore is expected to cause the expression of Ste11p targets. We thus defined Ste11p targets as those genes whose expression was significantly reduced in a ste11 delta mutant and significantly increased when ste11 is overexpressed in vegetative cells.<br> <br> This study looked at the effect of deletion or overexpression of ste11, and changes in gene expression after nitrogen starvation of h plus, h minus, h90fus1 and h90ste11 cells.

2006-09-22 | E-TABM-139 | biostudies-arrayexpress

Transcription profiling of fission yeast timecourse mcs6 mutant

2004-11-01 | E-MEXP-192 | biostudies-arrayexpress

Transcription profiling of fission yeast timecourse pmh1 mutant

2004-11-01 | E-MEXP-191 | biostudies-arrayexpress

Transcription profiling of fission yeast as telomeres shorten in trt1- strains, and to compare survivors with linear and circular chromosomes

Project description:Time course experiment to watch RNA expression profiles as telomeres shorten in trt1- strains, and to compare survivors with linear and circular chromosomes.

2005-03-01 | E-MEXP-201 | biostudies-arrayexpress

Transcription profiling of fission yeast cell cycle, elutriation 2

Project description:Cell cycle timecourse of fission yeast using a selective synchronisation method ? elutriation nr. 2

2004-04-01 | E-MEXP-55 | biostudies-arrayexpress

Transcription profiling of fission yeast cell cycle, elutriation 1

Project description:Cell cycle timecourse of fission yeast using a selective synchronisation method elutriation nr. 1

2004-04-01 | E-MEXP-56 | biostudies-arrayexpress

Transcription profiling of fission yeast wild type and yox1 mutant strains

Project description:Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).

2009-08-20 | E-TABM-646 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data