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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of B. thuringiensis 407 nprA::lacZ and an isogenic delta-nprR-nprX strain

ABSTRACT: B. thuringiensis 407 nprA::lacZ strain was compared with an isogenic delta-nprR-nprX strain to explore the effects of nprR-nprX deletion. NprR is a transition state regulator which is activated towards the end of the exponential phase by a part of the signaling peptide NprX. We made direct comparisons of RNA from the two strains collected at T1 and T3, one and three hours after the transition to stationary phase, respectively. T1 was thought to be the early phase of NprR activation, while T3 was thought to be around the peak of NprR activation. All samples are biological replicates, and dye swap was performed between the different comparisons.

ORGANISM(S): Bacillus thuringiensis Bt407

SUBMITTER: Karoline Faegri

PROVIDER: E-TABM-790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Necrotrophism is a quorum-sensing-regulated lifestyle in Bacillus thuringiensis.

Dubois Thomas T Faegri Karoline K Perchat Stéphane S Lemy Christelle C Buisson Christophe C Nielsen-LeRoux Christina C Gohar Michel M Jacques Philippe P Ramarao Nalini N Kolstø Anne-Brit AB Lereclus Didier D

PLoS pathogens 20120412 4

How pathogenic bacteria infect and kill their host is currently widely investigated. In comparison, the fate of pathogens after the death of their host receives less attention. We studied Bacillus thuringiensis (Bt) infection of an insect host, and show that NprR, a quorum sensor, is active after death of the insect and allows Bt to survive in the cadavers as vegetative cells. Transcriptomic analysis revealed that NprR regulates at least 41 genes, including many encoding degradative enzymes or p ...[more]

PMID: 22511867

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