Transcription profiling of Dictyostelium discoideum strains during growth and early development

ABSTRACT: Dictyostelium discoideum is an ideal organism to study SH2-domain mediated signal transduction because not only does it provide relatively simple model cell biological and developmental processes, but also possesses a highly streamlined repertoire of SH2-containing proteins. We studied one such novel protein, LrrB, by gene deletion and protein interaction experiments. A DNA microarray experiment carried out on growing cells reveals misregulation of a small set of vegetative genes in the lrrB knockout strain when compared to the parent, Ax2. Real time PCR experiments described in the accompanying manuscript extend these observations to show how this affects gene expression during early development, and that knockouts in another SH2 protein that interacts with LrrB show misregulation of some

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Gareth Bloomfield 

PROVIDER: E-TABM-803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress