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Chromatine immunoprecipitation of mouse thymus and HPC-7 cell line with anti-acetyl histone H3 antibody.

ABSTRACT: Histone modification ChIP-on-chip assays were performed in hematopoietic cell lines to identify potential cis-regulatory regions

ORGANISM(S): Mus musculus

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PROVIDER: E-TABM-882 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gfi1 expression is controlled by five distinct regulatory regions spread over 100 kilobases, with Scl/Tal1, Gata2, PU.1, Erg, Meis1, and Runx1 acting as upstream regulators in early hematopoietic cells.

Wilson Nicola K NK Timms Richard T RT Kinston Sarah J SJ Cheng Yi-Han YH Oram S Helen SH Landry Josette-Renee JR Mullender Joanne J Ottersbach Katrin K Gottgens Berthold B

Molecular and cellular biology 20100601 15

The growth factor independence 1 (Gfi1) gene was originally discovered in the hematopoietic system, where it functions as a key regulator of stem cell homeostasis, as well as neutrophil and T-cell development. Outside the blood system, Gfi1 is essential for inner-ear hair and intestinal secretory cell differentiation. To understand the regulatory hierarchies within which Gfi1 operates to control these diverse biological functions, we used a combination of comparative genomics, locus-wide chromat ...[more]

PMID: 20516218

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