Transcription profiling of Enterococcus faecalis during growth in urine
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ABSTRACT: Differences in gene content between three test strains were first assessed by comparative genomic hybridization using an E. faecalis V583 oligo array and with E. faecalis V583 as a reference strain . Transcriptional profiles of the same three test strains were then obtained through time series experiments over periods of 30min.
Project description:Comparative genomic hybridization of 15 strains isolated in European hospital environments and selected for CGH based on MLST sequence types
Project description:Comparative genomic hybridization of 9 Norwegian E. faecalis baby isolates with E. faecalis V583 as a reference strain using an E. faecalis V583 oligo array. Total gene content was analyzed by whole genome microarrays.
Project description:The transcriptional profiles of wild type Enterococcus faecalis V583 and two rex-deficient mutants (EF2638 and EF2933) were compared
Project description:The effects of NaCl on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 60min.
Project description:We have studied the effects of three different growth rate (0.05h (-1), 0.15h (-1) and 0.4h(-1) on the trancriptome of E. faecalis grown under energy limitation in chemostat culture
Project description:The effects of bovine bile and SDS on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 120min for cells treated with bile and the combination of SDS and bile, and 360min. for SDS.
Project description:Comparison of the transcription profile between Enterococcus faecalis V583 strain and its mutant having a constitutive SOS response
Project description:Gene expression of Enterococcus faecalis V583 in response to growth in 10% and 100% blood was examined by the use of of genome wide microarrays. Transcriptional profiles were obtained through time series experiments over periods of 60min (for 10% blood) and only after 30 min for growth 100% blood.
Project description:Changes in RNA levels during osmotic stress were investigated. Total RNA was extracted from a wild-type yeast strain before and after treatment with 0.4 M NaCl and the corresponding cDNAs were hybridazed on Tiling arrays. In particular, for all the intron-containing genes, the changes in the levels of intron signal in stressed cells related to the intron signal in the non-stressed cells, and the changes in the levels of exon signal in stresses cells related to the exon signal in non-stressed cells were investigated. The supplementary bar file contains the ratios between stress signals respect to non-stress signals, using the average of the 3 biological replicas. For the tiling array experiments, total RNA was extracted from exponentially YPD growing W303-1A cells before and after treatment for 15 min with 0.4M NaCl. The hybridization of tiling array Affymetrix (GeneChipM-BM-. S. cerevisiae Tiling 1.0R Array) with cDNA produced from total RNA, was carried out according to the Affymetrix protocol and using GeneChipM-BM-. Hybridization, Wash and Stain Kit (P/N 900720). The final data were obtained from the results of three independent biological experiments. High-density tiling arrays images were normalised and analysed using the Affymetrix Tiling Analysis Software; and were visualized by the Integrated Genome Browser (Affymetrix).
Project description:The transcriptional responses of class IIa bacteriocin resistance in 4 mutants of Enterococcus faecalis V583; three spontaneous pediocin PA-1 mutants; MOP1, MOP2 and MOP5 and one delta-mptD mutant called MOM1, studied by microarrays in E. faecalis V583.