RNAi profiling by array of human HEp-2 cells with siRNA-mediated depletion of RNase MRP protein components

ABSTRACT: To identify mRNA substrates for the human RNase MRP complex, we examined the effects of siRNA-mediated depletion of RNase MRP (and RNase P) on the transcriptome of HEp-2 cells. The expression of two RNase MRP protein components, hPop1 and Rpp40, was knocked-down and after 48 hours mRNA was isolated from these cells as well as from cells transfected with an siRNA targeting the GFP mRNA (siEGFP), which was used as a control. The expression levels of mRNAs were analyzed on a genome-wide scale using 21K microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-TABM-981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress