Transcription profiling of wild type G. sulfurreducens DL1 strain and mutant delta-sfrAB::kan, acetate-adapted strain using fumarate as an electron acceptor

ABSTRACT: 1. Summary: SfrAB, the soluble ferric reductase of Geobacter sulfurreducens, is a two subunit complex that can catalyze the NADPH-dependent reduction of chelated Fe(III) and is encoded in a two gene cluster in which the sfrB gene (GSU_0510) is 126 bp upstream of the sfrA gene (GSU_0509)[Kaufmann and Lovley (2001) J. Bacteriol. 183:4468-4476]. In order to gain insight into the physiological function of SfrAB, a knockout mutant was constructed by replacing a 3.6 kb stretch of sequence encompassing the C-terminal 72% of the SfrB and the N-terminal 80% of the SfrA coding sequences with a kanamycin resistance cassette via homologous recombination. The SfrAB knockout mutant (Delta sfrAB::kan) was found to be initially unable to grow in media in which acetate served as the sole electron donor.

ORGANISM(S): Geobacter sulfurreducens

SUBMITTER: Barbara Methe 

PROVIDER: E-TIGR-130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress