Transcription profiling of Geobacter sulfurreducens grown in batch culture with either ferric citrate or graphite anodes as the electron acceptor
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ABSTRACT: G. sulfurreducens (ATCC #51573) was obtained from the laboratory culture collection of Dr. Derek Lovley. Cells were grown under strict anaerobic conditions at 23 °C in batch culture. Both control and experimental populations were grown with 10mM acetate as the electron donor while Fe(III) as 55 mM ferric citrate served as the electron acceptor for the control populations and graphite anodes as the electron acceptor for the experimental populations. Controls populations were harvested at a concentration of 30 mM Fe(II). Fuel cell power output was monitored by measuring the voltage across a known resistance in the fuel cell and experimental populations were harvested when current production reached a steady state of 0.6 milliamperes. Cells were harvested at log phase by centrifugation at 4 °
ORGANISM(S): Geobacter sulfurreducens
SUBMITTER: Joseph White
PROVIDER: E-TIGR-5001 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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