Transcription profiling of mouse lymphoma L5178Y cells treated with Hydroxyurea

ABSTRACT: The multi-lab International Life Sciences Institute (ILSI) project on the application of genomics to risk assessment offered the unique opportunity to investigate the influence of variability within and between laboratories on identifying biologically relevant gene expression changes. We assessed the gene expression profiles of mouse lymphoma L5178Y cells treated with hydroxyurea (HU) in three independent studies from two different laboratories, Sanofi-Aventis and Procter and Gamble. Cells were dosed for 4 hr and harvested immediately at the end of the treatment or after a 20-hr recovery period. Cytotoxicity and genotoxicity were evaluated by standard assays. Statistical analysis of these data revealed that, while gene expression responses to HU treatment were markedly different at 4 hr vs

ORGANISM(S): Mus musculus

SUBMITTER: Sanofi Aventis Performer 

PROVIDER: E-TOXM-17 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress