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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray-based comparison of RSC, Condensin and Cohesin

ABSTRACT: Total mRNA was isolated from parallel cultures of two Matalpha clones of each of the 7 temperature sensitive strains: STH1ts, SMC2ts, SMC3ts, STH1ts SMC2ts, STH1ts SMC3ts, SMC2ts SMC3ts, and STH1ts SMC2ts SMC3ts. The two biological replicates were processed in parallel and one was conjugated to Cy3, the other one to Cy5 dye. A competitive Cy3-Cy5 hybridization was then performed against aliquots of a pool of mRNA obtained from several clones of the congenic wild type strain. This experimental strategy was chosen to permit horizontal comparisons across the seven mutant strains.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Anne-Marie Collin

PROVIDER: E-UMCU-21 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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