Chromatin immunoprecipitation of mouse polycomb group (PcG) proteins in embryonic stem cells

ABSTRACT: Polycomb group (PcG) proteins are highly conserved from flies to mammals and many of these factors have essential roles in early embryonic development. PcG proteins comprise two multimeric complexes, the Polycomb Repressive complexes 1 and 2 (PRC1 and 2), which have been shown to repress transcription through epigenetic modification of chromatin structure. To gain insight into the role of Polycomb in early development, we have identified PRC1 and PRC2 target genes in mouse embryonic stem (ES) cells using genome-scale location analysis. We found that PRC2 occupies many genes that encode key regulators of development including those encoding transcription factors and components of signaling pathways. These genes are repressed and contain nucleosomes methylated at lysine 27 on histone H3.

ORGANISM(S): Mus musculus

SUBMITTER: Elizabeth Herbolsheimer 

PROVIDER: E-WMIT-12 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress