A High Throughput Screen with a Clonogenic Endpoint to Identify Radiation Modulators of Cancer.

ABSTRACT: Clonogenic assays evaluate the ability of single cells to proliferate and form colonies. This process approximates the regrowth and recurrence of tumors after treatment with radiation or chemotherapy, and thereby provides a drug discovery platform for compounds that block this process. However, because of their labor-intensive and cumbersome nature, adapting canonical clonogenic assays for high throughput screening (HTS) has been challenging. We overcame these barriers by developing an integrated system that automates cell- and liquid-handling, irradiation, dosimetry, drug administration, and incubation. Further, we developed a fluorescent live-cell based automated colony scoring methodology that identifies and counts colonies precisely based upon actual nuclei number rather than colony ar

SUBMITTER: Gomes NP 

PROVIDER: S-EPMC10000021 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature