Establishment of a Real-Time PCR Assay for the Detection of Devriesea agamarum in Lizards.

ABSTRACT: (1) Background: Devriesea (D.) agamarum is a potential cause of dermatitis and cheilitis in lizards. The aim of this study was to establish a real-time PCR assay for the detection of D. agamarum. (2) Methods: Primers and probe were selected targeting the 16S rRNA gene, using sequences of 16S rRNA genes of D. agamarum as well as of other bacterial species derived from GenBank. The PCR assay was tested with 14 positive controls of different D. agamarum cultures as well as with 34 negative controls of various non-D. agamarum bacterial cultures. Additionally, samples of 38 lizards, mostly Uromastyx spp. and Pogona spp., submitted to a commercial veterinary laboratory were tested for the presence of D. agamarum using the esta

SUBMITTER: Brockmann M 

PROVIDER: S-EPMC10000032 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature