Effect of Maternal Gradient Nutritional Restriction during Pregnancy on Mammary Gland Development in Offspring.

ABSTRACT: We aimed to investigate the effect of different levels of nutritional restriction on mammary gland development during the embryonic period by gradient nutritional restriction in pregnant female mice. We started the nutritional restriction of 60 female CD-1(ICR) mice from day 9 of gestation based on 100%, 90%, 80%, 70% and 60% of ad libitum intake. After delivery, the weight and body fat of the offspring and the mother were recorded (n = 12). Offspring mammary development and gene expression were explored by whole mount and qPCR. Mammary development patterns of in offspring were constructed using Sholl analysis, principal component analysis (PCA) and regression analysis. We found that: (1) Mild maternal nutritional restriction (90-70% of ad libitum intake) did not affect offspring we

SUBMITTER: Dong X 

PROVIDER: S-EPMC10000074 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature