Validation of p53 Immunohistochemistry (PAb240 Clone) in Canine Tumors with Next-Generation Sequencing (NGS) Analysis

ABSTRACT:

Simple Summary

TP53 is a tumor-suppressor gene that is involved in cell cycle arrest, and its mutation is an event that is recognized to cause and contribute to carcinogenesis. Increased immunohistochemical expression of p53 in tumors has been associated with mutation of the gene; therefore, this can be an important tool to detect p53 anomalies. In this study, the expression of p53 was compared to NGS analysis in order to verify if p53 could predict TP53 gene mutation. The results indicate that with a 10% threshold, the sensitivity of immunohistochemistry was 60%, the specificity was 86.7%, and the accuracy was 76%. This suggests that we can expect up to 25% inaccurate predictions. Moreover, a substantial decrease in sensitivity was observed, moving the threshold to 50%. Overall,

SUBMITTER: Brunetti B 

PROVIDER: S-EPMC10000222 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature