PD-1⁺CD8⁺ T Cells Proximal to PD-L1⁺CD68⁺ Macrophages Are Associated with Poor Prognosis in Pancreatic Ductal Adenocarcinoma Patients.

ABSTRACT: Immune complexity status in the TME has been linked to clinical outcomes in pancreatic ductal adenocarcinoma (PDAC) patients. TME assessments with current cell marker and cell density-based analyses do not identify the original phenotypes of single cells with multilineage selectivity, the functional status of the cells, or cellular spatial information in the tissues. Here, we describe a method that circumvents these problems. The combined strategy of multiplexed IHC with computational image cytometry and multiparameter cytometric quantification allows us to assess multiple lineage-selective and functional phenotypic biomarkers in the TME. Our study revealed that the percentage of CD8⁺ T lymphoid cells expressing the T cell exhaustion marker PD-1 and the high expression of the ch

SUBMITTER: Yang X 

PROVIDER: S-EPMC10000394 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature