Multiparametric Profiling of Neutrophil Function via a High-Throughput Flow Cytometry-Based Assay.

ABSTRACT: Neutrophils are a vital component of the innate immune system and play an essential function in the recognition and clearance of bacterial and fungal pathogens. There is great interest in understanding mechanisms of neutrophil dysfunction in the setting of disease and deciphering potential side effects of immunomodulatory drugs on neutrophil function. We developed a high throughput flow cytometry-based assay for detecting changes to four canonical neutrophil functions following biological or chemical triggers. Our assay detects neutrophil phagocytosis, reactive oxygen species (ROS) generation, ectodomain shedding, and secondary granule release in a single reaction mixture. By selecting fluorescent markers with minimal spectral overlap, we merge four detection assays into one microtiter pla

SUBMITTER: Timmer KD 

PROVIDER: S-EPMC10000770 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature